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  • 产品名称:ZYMO EZ DNA Methylation-Gold Kit甲基化试剂盒

  • 产品型号:R5001
  • 产品厂商:Zymo
  • 产品价格:2251
  • 折扣价格:0
  • 产品文档:
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ZYMO EZ DNA Methylation-Gold Kit甲基化试剂盒 江苏弘麒现货供应 R5001 2-3天发货 期货2-3周发货 ZYMO EZ DNA Methylation-Gold Kit甲基化试剂盒
详情介绍:

ZYMO EZ DNA Methylation-Gold Kit甲基化试剂盒 

ZYMO EZ DNA Methylation-Gold Kit甲基化试剂盒 将DNA变性与亚硫酸氢盐转化过程变为一步,此步骤采用温度变性来取代先前操作步骤中使用氢氧化钠的化学变性,经过亚硫酸氢盐处理后试剂盒可大量回收到DNA。该试剂盒是基于胞嘧***与亚硫酸氢盐之间发生的使胞嘧***转化为尿嘧***的三步反应过程。ZYMO货号D5005 ZYMO EZ DNA Methylation-Gold Kit甲基化试剂盒 采用**的柱内脱磺酸技术来减少不必要的DNA沉淀,以便提供给研究人员更好的结果。试剂盒的设计可以减少模板降解和纯化过程中的DNA损失,并且完全转化未甲基化的胞嘧***。回收的DNA对于PCR、、测序、微阵列等下游分析是很理想的。整个过程在3小时内完成。

ZYMO EZ DNA Methylation-Gold Kit甲基化试剂盒 的优势:

(1)时间—全部操作可在3小时内完成

(2)转化效率—完全转化富含GC的DNA

(3)DNA回收效率—与特殊配制的转化缓冲液一起发挥作用的热变性可使在转化过程中*大限度的回收的DNA

 ZYMO EZ DNA Methylation-Gold Kit甲基化试剂盒 3小时内完全转化富含GC的DNA

DNA中的胞嘧***甲基化检测使用快速的温度变性步骤来取代其他试剂盒所采用的耗时的化学变性步骤。

相互紧密联系的DNA变性与亚硫酸氢盐处理步骤可使未甲基化的胞嘧***完全转化为尿嘧***的过程更为流畅。

通过**的spin-column技术,可一步完成DNA的纯化与脱硫,并且无DNA沉淀。

ZYMO EZ DNA Methylation-Gold Kit甲基化试剂盒

每次处理:500pg-2ug DNA

理想数值为:200-500ng


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表观遗传系列

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