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  • 产品名称:Anti-DMT1 antibody

  • 产品型号:ab55735
  • 产品厂商:Abcam
  • 产品价格:0
  • 折扣价格:0
  • 产品文档:
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简单介绍:
Anti-DMT1 antibody Abcam 位于英国的剑桥科学园,成立于1998年,专门生产和分销研究型抗体。我们的在线目录 (www.abcam.cn) 已有超过120,000 种抗体和试剂,并不断添加,供应予全球百多个国家。 Anti-DMT1 antibody
详情介绍:

Anti-DMT1 antibody

描述: Mouse monoclonal to DMT1
形态: Liquid
保存条件: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
纯度: Protein G purified
克隆性:
亚型: IgG2a
轻链类型: kappa
功能: Important in metal transport, in particular iron. Can also transport manganese, cobalt, cadmium, nickel, vanadium and lead. Involved in apical iron uptake into duodenal enterocytes. Involved in iron transport from acidified endosomes into the cytoplasm of erythroid precursor cells. May play an important role in hepatic iron accumulation and tissue iron distribution.
相关**: Defects in SLC11A2 are a cause of hypochromic microcytic anemia (HCMA)[MIM:206100]. The disease is characterized by an abnormal hemoglobincontent in the erythrocytes which are reduced in size. It may behereditary or acquired. Mutations in SLC11A2 are associated withprogressive liver iron overload and normal to moderately elevated serumferritin levels.
序列相似性: Belongs to the NRAMP family.
胞内定位: Endosome membrane.
浓度: 100 g at 0.36 mg/ml
宿主: Mouse
靶点: DMT1
测试型应用: Flow Cyt , IHC-P , WB , IHC-Fr more details
**原: <p>Recombinant fragment: MVLGPEQKMSDDSVSGDHGESASLGNINPYSNPSLSQSPG DSEEYFATYFNEKISIPEEEYSCF, corresponding to amino acids 1-66 of Human DMT1</p> <div> Run BLAST with Run BLAST with</div>
保存缓冲液: Preservative: None PBS, pH 7.2
数据库链接:
  • Entrez Gene: 4891 Human
  • Entrez Gene: 18174 Mouse
  • Omim: 600523 Human
  • SwissProt: P49281 Human
  • SwissProt: P49282 Mouse
  • Unigene: 505545 Human
  • Unigene: 234608 Mouse
别名:
  • DCT 1 antibody  Anti-DMT1 antibody
  • dct-1 antibody
  • DCT1 antibody
  • Divalent cation transporter 1 antibody
  • Divalent metal transporter 1 antibody
  • DMT 1 antibody
  • DMT-1 antibody
  • DMT1 antibody
  • FLJ37416 antibody
  • Natural resistance associated macrophage protein 2 antibody
  • Natural resistance-associated macrophage protein 2 antibody
  • NRAM2_HUMAN antibody
  • NRAMP 2 antibody
  • NRAMP2 antibody
  • OK/SW-cl.20 antibody
  • Slc11a2 antibody
  • Solute carrier family 11 (proton coupled divalent metal ion transporters) member 2 antibody
  • Solute carrier family 11 member 2 antibody
应用范围: {"Flow Cyt":"\n Flow Cyt: Use 1g for 10\n \n<sup>6</sup>\n\n cells.\n\n","IHC-Fr":"IHC-Fr: 1/1000.","IHC-P":"IHC-P: Use a concentration of 3 g/ml.","WB":"\n WB: Use a concentration of 1 - 5 g/ml.\n \n<br />\n\n \n<b>This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.</b>\n\n\n"}
品牌: abcam
规格: 100 g
  • Immunohistochemistry (Frozen sections) - Anti-DMT1 antibody (ab55735) Image courtesy Dr. Ruma Raha-Chowdhury (Cambridge University, United Kingdom)

    IHC-Fr image of DMT1 staining on mouse duodenum using ab55735 (1:1000). The sections were fixed with paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The slides were blocked using 10 % donkey serum for 1 hour at 24°C. ab55735 was diluted 1:1000 using 0.1% TritonX with 0.1x PBS- 10% Donkeys and incubated with the slides at 4°C for 24 hours. The secondary antibody used was donkey polyclonal against mouse IgG conjugated to Alexa Fluor 568 (1:1000)

    See Abreview

  • Concentration inion loading...
  • Western blot - DMT1 antibody (ab55735) Anti-DMT1 antibody

    Western blot against tagged recombinant protein immunogen using ab55735 DMT1 antibody at 1ug/ml. Predicted band size of immunogen is 33 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DMT1 antibody (ab55735)

    DMT1 antibody (ab55735) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium cancer.

  • Flow Cytometry-DMT1 antibody(ab55735)

    Overlay histogram showing SH-SY5Y cells stained with ab55735 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55735, 1g/1x10 6 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) ( ab96879 ) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] ( ab91361 , 1g/1x10 6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions. 
     

  • Immunohistochemistry (Frozen sections) - Anti-DMT1 antibody (ab55735)

    Immunohistochemistry (Frozen sections) - Anti-DMT1 antibody (ab55735) Image courtesy Dr. Ruma Raha-Chowdhury (Cambridge University, United Kingdom)

    IHC-Fr image of DMT1 staining on mouse duodenum using ab55735 (1:1000). The sections were fixed with paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The slides were blocked using 10 % donkey serum for 1 hour at 24°C. ab55735 was diluted 1:1000 using 0.1% TritonX with 0.1x PBS- 10% Donkeys and incubated with the slides at 4°C for 24 hours. The secondary antibody used was donkey polyclonal against mouse IgG conjugated to Alexa Fluor 568 (1:1000)

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DMT1 antibody (ab55735)

    DMT1 antibody (ab55735) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium cancer.

  • Western blot - DMT1 antibody (ab55735)

    Western blot - DMT1 antibody (ab55735)

    Western blot against tagged recombinant protein immunogen using ab55735 DMT1 antibody at 1ug/ml. Predicted band size of immunogen is 33 kDa

  • Flow Cytometry-DMT1 antibody(ab55735)

    Flow Cytometry-DMT1 antibody(ab55735)

    Overlay histogram showing SH-SY5Y cells stained with ab55735 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55735, 1g/1x10 6 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) ( ab96879 ) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] ( ab91361 , 1g/1x10 6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions. 






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